General Laboratory Safety
Policies, procedures, and practices common to all Penn State Laboratories and Research Locations.
All researchers must review the Work Specific Training Guide and all supplemental materials to stay informed and compliant with safety protocols.
EHS provides flexible training—online, in-person, or hands-on—and can customize courses to fit your team’s needs.
Compliance
Researcher Responsibilities
Faculty and Principal Investigators are responsible for lab safety, compliance, training, and oversight. They must:
- Model safe behavior and enforce safety policies (including SY01).
- Maintain safe lab conditions and ensure proper handling of hazardous materials.
- Conduct risk assessments and plan experiments to minimize exposure.
- Develop and document lab-specific safety procedures and training.
- Ensure all personnel complete required safety training.
- Secure lab materials and equipment from unauthorized access.
- Maintain records (e.g., LionSafe Risk Assessment, PPE Hazard Assessment).
- Collaborate with EHS and OPP to install and maintain safety equipment.
- Provide and maintain necessary lab and PPE equipment.
- Follow lab close-out procedures when relocating or closing labs.
- Keep spill kits appropriate to lab hazards.
- Conduct inspections and address safety issues promptly.
- Investigate incidents with EHS and implement corrective actions.
- Take immediate action to stop unsafe activities.
Delegation: PIs/Supervisors may delegate tasks but retain full responsibility for oversight and compliance.
Laboratory Personnel must conduct work safely and responsibly. They are expected to:
- Follow Penn State safety policies and procedures.
- Complete all required safety and work-specific training.
- Know how to access and use SDSs.
- Understand proper handling, storage, and disposal of hazardous materials.
- Be familiar with emergency procedures, PPE, fire equipment, and lab security.
- Report unsafe conditions, injuries, or incidents immediately to supervisors or EHS.
Laboratory Records
Researchers must maintain and make accessible the following records:
LionSafe Risk Assessment
- Complete and store in LionSafe.
- Print and post in the lab.
- Train staff and document training in the Work-Specific Safety Training Record.
PPE Hazard Assessment
- Complete and post in the lab.
- Train staff and record training.
Work-Specific Safety Training Records
- Track who was trained, what was covered, and when.
- Include refresher sessions.
- Keep printed records accessible.
Biological Inventory
- List all biological materials, including genus/species and storage locations.
- Note presence of variants or clones.
- Post and keep accessible.
Chemical Inventory
- Use LionSafe to update chemical lists annually or when changes occur.
- Print and post in the lab.
Safety Data Sheets (SDS)
- Maintain SDS for all chemicals.
- Use LionSafe to manage and update.
- Keep printed copies accessible.
Standard Operating Procedures (SOPs)
- Create SOPs for lab operations.
- Train staff and document training.
- Post and keep accessible.
Satellite Waste Accumulation Area Inspections
- Inspect weekly and label containers.
- Keep inspection records for three years.
Eye Wash Flushing Records
- Flush weekly.
- Record flushes visibly near the station.
Protocol Registrations
- Store committee approvals (IBC, IACUC, IRB, UIC) in CATS.
Permits and Licenses
- Maintain current permits/licenses (CDC, USDA, DEA, etc.) per regulations.
Self-Inspections
- Conduct for new or relocated labs.
- Document inspections and corrective actions.
LionSafe Risk Assessment, LRA
Contributing to a comprehensive inventory of hazards at Penn State, and ultimately leading to a safer workplace and understanding of University safety requirements.
New PI Checklist (coming soon)
Protocol Registration (coming soon)
Chemical Inventory
Maintaining an accurate chemical inventory is a fundamental requirement that supports both regulatory compliance and laboratory safety. It is a mandated practice for the proper use, handling, and storage of hazardous materials.
Learn more about Chemical Inventories at Penn State on the Chemical Safety page.
Audits and Inspections
The self-inspection of laboratories is required when a new laboratory begins operations or when a laboratory relocates from one lab to another. The Self-Inspection Checklist for Laboratories (link) should be used for these inspections. Results of self-inspections should be maintained with laboratory records and be made available upon request.
Note: The checklist may also be used at any time to evaluate safety and compliance in work areas.
Inspection Checklists:
Download the Laboratory and Research Safety Self Inspection Form on SharePoint.
Download the Laboratory and Research Safety Self Inspection Guide on SharePoint.
Signage (coming soon)
Laboratory Waste (coming soon)
Emergency Showers and Eye Washes
Download the Emergency Showers and Safety Showers at Penn State Procedure on SharePoint.
Download the Weekly Eyewash Schedule Poster on SharePoint.
Incidents, Accidents, and Emergencies (coming soon)
Standard Research Safety Practices
Develop Knowledge and Skills
Know the Hazards: Understand risks in your lab and work area before starting.
Complete Required Training: Finish all safety courses before beginning lab work. Take annual refreshers as needed.
Update Work-Specific Training: Refresh training when project scope, protocols, or materials change.
Work Within Your Training: Only perform tasks you’re trained and authorized to do.
Plan with Safety in Mind: Include risk assessments when designing experiments or writing SOPs.
Review Safety Info Before Starting: Read protocols, SOPs, SDSs for chemicals, pathogen data sheets, and equipment manuals.
Dress appropriately for the work area
Follow University requirements for proper laboratory attire outlined in the PPE Program and PPE Hazard Assessment.
Prepare for Incidents, Accidents, and Emergencies
Emergency Preparedness
- Know incident response procedures and how to call for help. Locate the nearest phone.
- Identify and understand how to use safety equipment: emergency showers, eye washes, fire extinguishers.
- Keep emergency equipment and exit paths clear.
- Flush eyewash stations weekly and log the date.
- Know where fire alarm pull stations are and at least two exits. Follow posted evacuation plans and participate in annual drills.
Reporting and Safe Practices
- Report unsafe conditions, incidents, and accidents to your supervisor and EHS immediately.
- Avoid working alone, especially during unfamiliar or hazardous procedures.
Follow Good Laboratory Hygiene and Housekeeping Practices
Prevent Contamination and Exposure
- Remove PPE before leaving the lab. Never touch surfaces (e.g., door handles, elevator buttons) with gloves.
- Wash hands after removing gloves and before exiting.
- Aerosols from lab work can contaminate surfaces and PPE—proper glove removal and handwashing reduce risk.
- Wash hands with soap and water for 20–30 seconds; use a paper towel to turn off the faucet.
Protect Personal Items
- Secure phones, tablets, and earbuds. Use only after removing PPE and washing hands.
Minimize Exposure Risks
- Follow restrictions in areas with hazardous materials: no eating, drinking, chewing, applying cosmetics, or mouth pipetting.
- Always use mechanical pipetting devices.
- Never store food or drinks in labs or areas with hazardous materials or waste.
Maintain a Safe Environment
- Keep floors dry and clean.
- Keep aisles, hallways, and stairs clear—never use them for storage.
- Clean work surfaces regularly.
- Do not disable safety guards on equipment.
- Maintain and certify lab equipment as required. Label malfunctioning items “out of service.”
- Dispose of trash and lab waste properly. Do not overfill containers.
Establish Routine Work Hours
Establish Regular Lab Hours: Set standard lab hours (Monday–Friday, 8 a.m.–5 p.m.) when supervisors and staff are available. Conduct hazardous or first-time procedures during these hours.
Limit Extended Hours Work: Evening, weekend, and holiday work is riskier due to limited support. Maintain a schedule of who is working during these times to ensure safety.
Be Alert and Aware of your Surroundings
Work Only When Alert: Avoid lab work if you're tired, sick, drowsy, or under the influence of substances that impair alertness.
Stay Aware: Watch your surroundings, especially in shared lab spaces. Match PPE levels before approaching others using enhanced protection.
Know the Hazards: Understand the risks in your lab and work area before starting.
Avoid Working Alone
Avoid working alone. EHS strongly discourages it.
If necessary, get prior approval from your PI or supervisor. Approval should be limited to trained, experienced individuals and exclude high-risk activities.
Notify a coworker of your location, task, and expected return time. Let them know when you leave. If they don’t hear from you, they should check in to ensure you're safe.
Manage Experiments that Run Unattended
Assess Risks: Before leaving an experiment unattended, conduct a thorough risk assessment. Consider power loss, heating/cooling failure, runaway reactions, ventilation, containment, and need for backup systems or remote monitoring.
Use Safety Controls: Implement automatic shut-offs, pressure relief valves, and secondary containment for hazardous materials.
Post Clear Signage: Include experiment details, expected conditions, return time, and contact info for the owner and backup contact.
Ensure Awareness: Inform co-workers and supervisors. At least one other person must know how to safely shut down the experiment.
Never Leave High-Risk Experiments Unattended: If there's potential for uncontrolled reactions or hazardous releases, mitigation must be in place.
Critical Operations Shut-down
Prioritize personal safety—leave the lab immediately during emergencies.
If safe to do so, quickly shut down active hazards (e.g., turn off gas, unplug heating devices).
Design experiments with emergency shut-off plans or automatic controls.
Maintain Security and Access Control
Restrict access to toxic and corrosive chemicals at all times.
Lock labs and storerooms when unattended.
Use locked cabinets or other secure storage methods.
Follow federal regulations for storing highly hazardous substances.
Lab Security and Access
It is imperative that researchers implement appropriate security precautions to prevent unauthorized individuals from gaining access to laboratory materials and equipment.
Security Procedures:
- Identify potential security risks in the laboratory (e.g., doors left open, doors left unlocked when the room is unattended, or unsecured hazardous substances storage areas).
- Develop and implement laboratory security procedures to prevent unauthorized entry to the laboratory and access to hazardous substances
- Develop and implement laboratory access restrictions to protect the health and safety of individuals entering the laboratory. Hazards and access restrictions should be clearly indicated at the entrance to all laboratories.
- Train laboratory personnel to implement security procedures.
To secure the laboratory, identify potential security risks, establish and enforce access restrictions and procedures to prevent unauthorized entry and exposure to hazardous substances, clearly post hazard warnings at all entrances, and ensure all personnel are trained in these protocols.
The following security procedures must be followed in all laboratories:
- Keep doors closed at all times and locked when no authorized personnel are present.
- Do not leave hazardous substances unattended or unsecured at any time.
- Restrict access to freezers, refrigerators, storage cabinets, and other equipment where hazardous substances are stored.
- Limit laboratory access to approved laboratory personnel who are properly trained with regard to the hazards present in the laboratory and the work they will perform.
- Challenge or question unfamiliar or suspicious individuals that gain access to restricted areas or to the laboratory. Report the incidents to University Police. Escort visitors to and from the laboratory.
- Report any missing inventory and all acts of vandalism, theft, or suspicious activities to University Police.
Restricted Access for Visitors
Visitors should not be allowed to enter laboratories unattended and should be escorted to and from the laboratory by Penn State personnel. Prior to allowing visitors to tour or observe in a laboratory, the supervising employee must conduct a basic safety orientation, including both general safety information and any hazards particular to the location. A signed Acknowledgement for Risk for Laboratories form is required for visitors to enter a laboratory. The Principal Investigator or Laboratory Supervisor should maintain documentation of dates of training, a list of attendees. This requirement does not include trained Penn State employees, visiting scientists who must attend EHS training, or inspectors.
Restricted Access for Support Services Staff
Visitors must be escorted by Penn State personnel and receive a safety orientation before entering a laboratory. A signed Acknowledgement of Risk form is required, and the Principal Investigator or Supervisor must document training dates and attendees; trained Penn State employees, visiting scientists attending EHS training, and inspectors are exempt from this requirement.
All support services staff (e.g., housekeeping, facilities management, police, Information Technology Staff, etc.) must receive appropriate training prior to entering laboratories or laboratory support rooms. Once trained, support services staff may enter laboratories and laboratory support rooms.
For non-restricted laboratories and laboratory support rooms, support services staff must notify the unit of non-routine services (e.g., mopping and waxing floors, light bulb replacement, and equipment inventory) at least 10 university working days in advance of when the work is to occur. Researchers may request that services be scheduled at a time that does not interfere with ongoing laboratory operations or critical experiments. Support services staff have been instructed that they need not perform any services which make them uncomfortable; specifically, housekeeping staff has been instructed that they should not clean pools of liquid on laboratory floors.
Standard Operating Procedures (coming soon)
SOPs are detailed, written procedures that identify hazards and outline safe practices before an experiment begins. They help prevent health and safety risks by planning each step with safety in mind.
Write SOPs clearly and concisely. Include enough detail so someone with limited experience can follow them safely. Tailor them to your facility and specific procedures.
Templates Available
- Standard SOP Template
- High-Hazard SOP Template
- Chemical SOPs
Special Considerations
Laboratory Design and Renovation (coming soon)
Coming Soon.
Laboratory Cleanouts and Closeouts
Download the Laboratory Internal Move Guide document on SharePoint
Download the Laboratory External Move Guide document on SharePoint
Download the Laboratory Closeout Criteria document on SharePoint
Laboratory Equipment Clearance
Download the Laboratory Equipment Clearance Form on SharePoint.
Minors in Research
To protect their health and safety, minors are restricted from work involving certain hazardous materials. All proposals for minors volunteering in a laboratory or research setting must first be approved through the Youth Program Compliance Office. Proposals can be submitted with the Single Youth Program Registration Form and must include a risk assessment that details the work performed by the minor and any hazards to which the minor may be exposed. EHS conducts a review of proposals to verify that the work is appropriate and safety controls are sufficient.
EHS Resource:
Download the Restrictions for Minors in Research EHS document on SharePoint.
Websites to Reference:
Electrical Safety in Laboratories
Lab Electrical Safety: For Modified/Lab-Made Equipment Review the Energized Electrical Safety Program
Requirements/ Guidelines for the Energized Electrical Safety Program include:
- Risk Assessment for Modified/Lab-Made Electrical Equipment
- Appendix A - Risk Assessment for Modified/Lab-Made Electrical Equipment
- Appendix B - Risk Assessment for Modified/Lab-Made Electrical Equipment - Qualified Person Approval Form
- Multimeter - Safe Use Requirements Guide
- Example SOP - for lab-modified/lab-made electrical equipment
Adult Volunteers in Research and Indemnification
Download the Adult Volunteers in Penn State Laboratories document on SharePoint.